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Protein Dynamics, Glass Transition

Dr. Dr.habil. Wolfgang Doster

Physikdepartment
Technische Universität München
James-Franck-Straße 1
D-85747 Garching, Germany  wdoster@ph.tum.de 



The main research topic of my group is the neutron scattering analysis of protein dynamics and of hydration water on a pico-second time scale covering also extreme conditions of low temperature and high pressure. The first interpretation of the anharmonic onset in terms of a resolution dependent dynamical transition was given in 1986:
W. Doster, Glass Transition of protein hydration water and flexibility of myoglobin in:
Structure and Function of Biomolecules, first EBSA Workshop, Springer 1986, p. 34-38. PDF


Recent Articles:

W. Doster, Comment on "puzzle of the protein dynamical transition" by Magazu et al., J. Phys. Chem. B, submitted.
PDF

S. Longeville and W. Doster in "Dynamics of Softmatter, Neutron Applications" (2012) Springer,
Chapter 8: Protein Dynamics and Function
PDF

R. Gebhardt, N. Takeda, U. Kulozik and W. Doster
Structure and stabilizing interactions in casein micelles probed by
high pressure light scattering and FTIR, J.Phys.Chem. B (2011) 115:2349-2359.
PDF

W. Doster: The two-step scenario of the protein dynamical transition
Journal of Non-Crystalline Solids 357 (2011) pp. 622-628
DOI information: 10.1016/j.jnoncrysol.2010.08.002
PDF

W. Doster, S. Busch, M.A. Appavou, J. Wuttke and H. Scheer,
The dynamical transition of protein hydration water,
Phys. Rev. Lett. 104:098101 (2010) PDF

BBA Special Edition: Protein Hydration (2010) Cover PDF

W. Doster and Th. Gutberlet
Editorial: Protein-Water Energy Seascapes
BBA Proteins and Proteomics, Special Edition: Protein Hydration (2010)
PDF

W. Doster,
The Protein-Solvent Glass Transition,
BBA Proteins and Proteomics, Special Edition: Molecular Dynamics and Protein Hydration 1804 (2010) 3
PDF


A. Gaspar, S. Busch, M.S. Appavou, W. Häusler, Robert Georgii, Yixie Su and W. Doster
Using polarization analysis to separate the coherent from incoherent scattering from proteins
BBA Proteins and Proteomics, Special Edition: Protein Hydration, 1804(2010) 76 PDF

W. Doster:
Comment on protein phase diagrams, the physics behind their elliptical shape,
J. Chem. Phys, (2009) 130: 087101, (PDF)


W. Doster

Why protein phase diagrams are not related to protein conformational disorder,
PNAS (2008) published online. PDF

W. Doster
The dynamical transition of proteins, concepts and misconceptions
European Biophysics Journal (2008) 37:591 PDF



Teaching (German)

W. Doster, Lehrerfortbildung in Bayreuth: Die Metamorphosen des Wassers,
woher die Schwierigkeiten mit der Entropie kommen und wie sie gelöst werden könnten PDF

Wie erzeugt man Energie? Physik kompakt für Anfänger in 30 min
PDF

Main Interests:

  • picosecond structural dynamics and protein function
  • glass transition and mode coupling theory
  • thermodynamics of biomolecular solutions
  • protein-solvent interactions, hydration water and mixed solvents
  • dynamical transition and the role of viscosity in structural dynamics
  • energetics of reactions, mechanism of ligand binding to heme proteins
  • protein diffusion in crowded solutions and biological cells
  • critical association of proteins, amyloid fibres
  • structure and stability of casein micelles under pressure
  • pressure-induced folding/unfolding of proteins
  • new applications of dynamic neutron scattering to biomolecules: pressure and elastic resolution spectroscopy
  • Experimental Techniques:

  • dynamic neutron scattering (FRM2 Munich neutron source)
  • dynamic light scattering (PCS)
  • high pressure techniques: optical and diamond anvil cells
  • infrared-spectroscopy (FTIR)
  • flash photolysis, time resolved spectroscopy

  • by Wolfgang Doster, last changes: Dec. 29th 2011